phosphorylated iκb α  (Cell Signaling Technology Inc)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Fibroblast Growth Factor 20 Attenuates Colitis by Restoring Impaired Intestinal Epithelial Barrier Integrity and Modulating Macrophage Polarization via S100A9 in an NF-κB-Dependent Manner

doi: 10.1016/j.jcmgh.2025.101486

Figure Lengend Snippet: FGF20 inhibits NF-κB pathway through S100A9 in DDS-induced mice. ( A ) KEGG Enrichment Analysis of proteomics data from the colonic tissue of FGF20 KO and WT mice with or without colitis. ( B ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα from FGF20 KO and WT mice with or without colitis (n = 6). ( C ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα contents in AAV-FGF20- or AAV-GFP-treated mice with or without colitis. ( D ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα contents in FGF20 KO mice and WT mice with colitis, treated with paquinimod or vehicle (n = 6). ( E ) Immunoblot analysis of colonic p-P65, P65, p-IκBα, and IκBα contents in AAV-FGF20-treated mice and AAV-GFP-treated mice with colitis, treated with AAV-S100A9 or not (n = 6). Data are represented as mean ± SEM; ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001.

Article Snippet: FGF20 (1/500, SC-373927, Santa CruzA), Bcl2 (1/1000, AB182858, Abcam), Bax (1/1000, AB32503, Abcam), S100A9 (1/500, SC-53187, Santa Cruz), S100A8 (1/500, SC-48352, Santa Cruz), phosphorylated (p) -IκBα (1/500, SC-8404, Santa Cruz), p-P65 (1/500, SC-136548, Santa Cruz), IκBα (1/1000, 4814S, CST, USA), P65 (1/1000, 4814S, CST), TNF-α (1/1000, AF7014, Affinity, China), IL6 (1/1000, 12912S, CST), FGFR1 (1/1000, bs-0230R, Bioss, China), FGFR2 (1/1000, bs-0675R, Bioss), FGFR3 (1/2000, MA5-31142, ThermoFisher), FGFR4 (1/1000, bsm-61509R, Bioss), GAPDH (1/10000, HRP-60004, Proteintech, China), and Beta-Tubulin (1/5000, 10094-1-AP, Proteintech).

Techniques: Western Blot

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Fibroblast Growth Factor 20 Attenuates Colitis by Restoring Impaired Intestinal Epithelial Barrier Integrity and Modulating Macrophage Polarization via S100A9 in an NF-κB-Dependent Manner

doi: 10.1016/j.jcmgh.2025.101486

Figure Lengend Snippet: FGF20 inhibits M1-like macrophage polarization in NF-κB-dependent manner in vitro. Primary peritoneal macrophages were isolated from WT mice and FGF20 KO mice and used for subsequent in vitro experiments. ( A ) Immunoblot analysis of expression of iNOS, TNF-α, IL6, S100A9, p-P65, P65, p-IκBα, and IκBα in LPS-stimulated primary peritoneal macrophages, treated with or without BAY11-7082 (n = 3). ( B ) Representative immunofluorescence images and its fluorescence densities of LPS-stimulated primary peritoneal macrophages, treated with or without BAY11-7082 (n = 6). Data are represented as mean ± SEM; ∗∗ P < .01, ∗∗∗ P < .001.

Article Snippet: FGF20 (1/500, SC-373927, Santa CruzA), Bcl2 (1/1000, AB182858, Abcam), Bax (1/1000, AB32503, Abcam), S100A9 (1/500, SC-53187, Santa Cruz), S100A8 (1/500, SC-48352, Santa Cruz), phosphorylated (p) -IκBα (1/500, SC-8404, Santa Cruz), p-P65 (1/500, SC-136548, Santa Cruz), IκBα (1/1000, 4814S, CST, USA), P65 (1/1000, 4814S, CST), TNF-α (1/1000, AF7014, Affinity, China), IL6 (1/1000, 12912S, CST), FGFR1 (1/1000, bs-0230R, Bioss, China), FGFR2 (1/1000, bs-0675R, Bioss), FGFR3 (1/2000, MA5-31142, ThermoFisher), FGFR4 (1/1000, bsm-61509R, Bioss), GAPDH (1/10000, HRP-60004, Proteintech, China), and Beta-Tubulin (1/5000, 10094-1-AP, Proteintech).

Techniques: In Vitro, Isolation, Western Blot, Expressing, Immunofluorescence, Fluorescence